Funded PhD position (M/F) in cell/developmental biology, engineering and biophysics
- Montpellier
- CDD
- Temps-plein
Bénédicte DELAVAL's team, Centrosome Cilia and Pathologies, CRBM CNRS, Montpellier, France
Doctoral school: CBS2 (Chemical and Biological Sciences for Health) University of Montpellier
Co-direction: Bénédicte DELAVAL, CNRS Biology, CRBM Montpellier - Caterina TOMBA, CNRS Engineering, INL LyonFunding and work context: This thesis project is funded for 3 years by the CNRS through the MITI interdisciplinary programs. It will be carried out in Montpellier in Bénedicte DELAVAL's team at the Centre de Recherche en Biologie cellulaire de Montpellier (CRBM), in close collaboration with Caterina TOMBA (Institut des Nanotechnologies de Lyon). The PhD student will have access to laboratory equipment, microscopy facility (MRI) and aquatic models for research (ZEFIX) in Montpellier, as well as clean room and L2 (Nanolyon). Travel to Lyon will be required for the PhD student's training in micro-fabrication, as well as for consortium meetings.FUNDED PhD Position ( 3 years, CNRS funding for interdisciplinary programs -MITI)Title
Contribution of topographical constraints to kidney pathophysiological organizationInterdisciplinary thesis project. Despite lots of efforts to identify the origin of kidney diseases, the contribution of tissue topographical properties to renal pathophysiological organization remains poorly explored. Animal models are widely used, but remain complex systems that do not allow the dynamic study of tissues at the cellular scale in modular et standardized topographies. As for cellular studies on flat substrates, they do not take into account the naturally curved shape of the renal epithelial tubule. The thesis project proposed here combines cell biology and bioengineering. It consists in developing micro-fabricated 3D substrates that mimic the dynamic topographical constraints of the renal tubule, compatible with cell culture (renal MDCK cells and zebrafish renal tubule cells ex-vivo). These systems will be used to study how static or dynamic changes in tissue curvature influence the organization and integrity of the renal epithelium. High-resolution live microscopy will allow to focus on the organization and geometry of cell divisions. This project will lead to the implementation of a new device for ex vivo study of zebrafish renal tubules, and will allow to tackle, from a biophysical angle, the question of renal pathophysiology.Role of the PhD student. He/she will be trained in 3D micro-fabrication (Team C. Tomba, Lyon) and will use these substrates to characterize the impact of curvature on cell division in the Delaval team (CRBM, Montpellier). He/she will be in charge of MDCK cell culture, microscopy, quantification and biophysical analyses. He/she will also set up ex vivo cultures of zebrafish cells on structured 3D substrates and will participate in the optimization of micro-fabrication devices.Candidate profile. We are looking for motivated and enthusiastic candidate to work on an interdisciplinary project at the interface between cell biology, developmental biology, engineering and biophysics. Solid experimental knowledge and skills in cell biology, tissue morphogenesis, imaging/image analysis, micro-fabrication and biophysical analyses will be positively considered in the selection of candidates. Previous experience with the zebrafish model is not required to apply, but a strong interest in using this model in research will be positively considered. Good communication and team work skills will be required. Level required: Master 2 or equivalent validated before October 2024.Keywords: Cell division, tissue morphogenesis, renal epithelial tubule, tissue curvature, imaging, 3D micro-fabrication, biophysics.Applications will be done directly on the CNRS job offer website.
You will be asked to provide a CV, a cover letter and the names of 3 referees who could provide recommendation letters.
For any question, please contact: and caterina.tomba@ univ-lyon1.fr.RequirementsResearch Field Biological sciences Education Level Master Degree or equivalentResearch Field Biological sciences Education Level Master Degree or equivalentLanguages FRENCH Level BasicResearch Field Biological sciences Years of Research Experience NoneResearch Field Biological sciences » Biology Years of Research Experience NoneAdditional InformationAdditional commentsThis thesis project is funded for 3 years by the CNRS through the MITI interdisciplinary programs. Website for additional job detailsWork Location(s)Number of offers available 1 Company/Institute Centre de Recherche en Biologie cellulaire de Montpellier Country France City MONTPELLIER GeofieldWhere to apply WebsiteContact CityMONTPELLIER WebsiteSTATUS: EXPIRED
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