Open Rank Laboratory Sciences Professional - Pediatric Cardiology
University of Colorado
- Colorado
- $45,000-57,240 per year
- Permanent
- Full-time
- Will work on projects studying mechanisms of heart failure in congenital heart disease.
- Will learn a variety of wet-lab experiments, including several molecular biology techniques, high-resolution mitochondrial respirometry, cardiomyocyte isolation and culture, immune cell isolation and culture, and assays of inflammation.
- Will develop proficiency in collecting, recording, analyzing, and presenting data.
- Will learn mouse/rat IP and SQ injections.
- May assist in the training of new team members on laboratory protocols and operations.
- May assist the principal investigator with the development and writing of research protocols.
- May assist the Principal Investigator with the development and writing of manuscripts and presentations, including as a credited author where appropriate.
- Processing human whole blood samples, including isolation of peripheral blood mononuclear cells, plasma, and serum.
- In vitro murine primary cardiomyocyte isolation and culture.
- In vivo murine animal model work (mouse/rat), including injections, dissections, survival surgeries, and termination procedures.
- Functional mitochondrial bioenergetics analysis.
- Organization and cataloging of human samples, antibodies, and chemicals.
- Laboratory supply ordering and invoice tracking.
- RNA, DNA, and protein extraction.
- Reagent preparation.
- Immunological assays.
- Protein and RNA quantification (Western blotting, proteomics, RT-qPCR, RNAseq).
- ELISA and enzymatic activity assays.
- Data analysis and presentation.
- Bachelor’s degree in biology, molecular biology, chemistry, genetics, or related field.
- Substitution: A combination of education and related technical/paraprofessional experience may be substituted for the bachelor’s degree on a year-for-year basis.
- Bachelor’s degree in biology, molecular biology, chemistry, genetics, or related field.
- Substitution: A combination of education and related technical/paraprofessional experience may be substituted for the bachelor’s degree on a year-for-year basis.
- One (1) year professional-level research experience.
- Experience using rodent models.
- Cell culture experience.
- Training in molecular techniques with a good theoretical understanding and experience in the interpretation of results.
- Laboratory management skills.
- Two (2) years of professional-level research experience.
- Proficient knowledge of basic laboratory techniques including safety procedures and techniques.
- Ability to communicate effectively, both in writing and orally.
- Ability to establish and maintain effective working relationships with employees at all levels throughout the institution.
- Outstanding customer service skills
- Demonstrated commitment and leadership ability to advance diversity and inclusion.
- Knowledge of basic human anatomy, physiology, and medical terminology.
- Ability to interpret and master complex research protocol information.