M/F, Post-doc offer in Biotechnology, Development of biotechnological tools for the expression and folding of recombinant proteins

  • Toulouse
  • CDD
  • Temps-plein
  • Il y a 18 jours
Offer DescriptionThe purification of recombinant proteins is a major issue for both industry and academic laboratories, and failure to successfully purify soluble recombinant proteins that have a tendency to aggregate often halt promising projects. In agreement with their roles in the prevention of protein aggregation, co-overexpression of molecular chaperones, like DnaK, GroEL or SecB, is often applied when purifying aggregation-prone recombinant proteins in bacteria. Yet, the results are not always satisfactory and there is a strong need for the development of pioneering methods to directly specialize (or target) molecular chaperones towards selected aggregation-prone proteins. This project aims to develop such innovative tools, based on the use of recently discovered bacterial toxin-antitoxin systems, to selectively improve the folding and activity of difficult recombinant proteins expressed E. coli, the bacterial work horse for protein expression.
The work will mainly require skills in genetics and molecular biology but experience with protein expression and purification, or high-throughput screens will also be appreciated. The project is supported by Toulouse White Biotechnology (TWB; ) and some of the proposed experiments will be performed at the genetic engineering platform of TWB. Therefore, the candidate will need to be well organized, independent and capable of easily interacting with colleagues with different backgrounds.-Development and implementation of new genetic tools
-Protein solubility tests
-Protein expression in bacteria
-High-throughput screeningThe work will be mainly performed at the LMGM/CBI inToulouse. Part of the work will be carried out under at Toulouse White Biotechnology (TWB) and followed by industrial partners. The project is one year + one year contract (with the go/no go for a second year at the end of the first year).RequirementsResearch Field Biological sciences Education Level PhD or equivalentLanguages FRENCH Level BasicResearch Field Biological sciences » Biology Years of Research Experience 1 - 4Additional InformationEligibility criteriaThe candidate will have obtained a PhD and will have less than 2 years of experience as a postdoctoral fellow. Experience in bacterial genetics, molecular micro/biology, recombinant protein expression or molecular chaperones will be appreciated. She/he should be fluent in English, ready to work under confidentiality and committed to innovation and biotechnological applications.
Please attach your CV, e-mail addresses of two possible references, as well as a short letter of motivation.Website for additional job detailsWork Location(s)Number of offers available 1 Company/Institute Laboratoire de Microbiologie et de Génétique Moléculaires Country France City TOULOUSE GeofieldWhere to apply WebsiteContact CityTOULOUSE WebsiteSTATUS: EXPIRED

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