Laboratory Specialist Intermediate or Senior, Engel lab, Department of Pediatrics (UVA)
University of Virginia
- Charlottesville, VA
- Permanent
- Full-time
- Develop, implement, and maintain databases of tools and reagents necessary for laboratory operations
- Develop, deploy and maintain standard protocols and systems that longitudinally catalogue laboratory work products
- Maintain archive of research outputs (research data and datasets, presentations, grant applications, progress reports, etc.) for shared use by laboratory members.
- Serve as the point person for all laboratory reagent ordering, equipment acquisitions and maintenance.
- Work with the principle investigator to monitor laboratory expenditures and burn rates.
- Prepare, maintain and renew research regulatory protocols such as institutional biosafety and animal care and use to insure seamless research operations.
- Ensure and track compliance with all training requirements among laboratory personnel.
- Orient and train new laboratory members on safe laboratory practices, laboratory citizenship, and expectations regarding use of shared laboratory materials and equipment.
- Ensure compliance with all laboratory safety protocols/regulations and maintain logs.
- Prepare and maintain schedule of laboratory meetings, presentations, and journal clubs.
- In addition to the above job responsibilities, other duties may be assigned.
Lab Specialist Intermediate:
Education: Bachelor's degree.
Experience: 6 years of experience in a lab technician role. Master's degree may substitute for required experience. 9 years of experience accepted in lieu of degree.Lab Specialist Senior:
Education: Bachelor's degree.
Experience: 9 years of experience in a lab technician role. May substitute with Master's or Terminal degree plus 4 years of experience in a lab technician role and supervisory experience. 12 years of experience accepted in lieu of degree.Required knowledge, skills and abilities:
- Excellent communications skills, both verbal and written.
- Ability to use common computer programs routinely used in laboratory settings.
- Ability to learn basic bioinformatic and computational skills
- Ability to learn mass spectral analysis of proteins to quantize components of protein complexes under varied experimental conditions.
Experience with a diverse array of cell biology, molecular biology and protein biochemistry techniques including:
- Culturing of adherent and suspension cells using aseptic technique.
- Transient and stable transfection of cultured cells.
- Production and transduction of retro/lentivirus into cell lines and primary cells.
- RNAi-mediated knockdown and CRISPR-mediated knockout in cultured cells.
- Subcloning, site-directed mutagenesis of gene expression constructs and propagation of constructs in bacteria.
- Qualitative and quantitative PCR and RT-PCR.
- DNA, RNA and protein isolation and quantitation from cells and tissues.
- Western blotting, immune precipitation, co-precipitation and protein purification.
- Microscopy, including bright field, phase contrast and fluorescence techniques.
- In vitro and in vivo viability and efficacy studies.
Process for Internal UVA Applicants: Please apply through your Workday Home page, search “Find Jobs”, and search for “R0038916”.Process for External Applicants: Please visit UVA job board at and search for “R0038916”.Complete an application online with the following documents:
- CV/Resume
- Cover letter
- Provide 3 letters of recommendation. Please address letters of reference to: